A tree health status scoring campaign was carried out in 2020 on all OPTMix plots on sessile oak and Scots pine tree samples. We used the DEPERIS protocol developed by the French Forest Health Department. A new scoring campaign was carried out in summer 2021. The first analyzes show an increase in the percentage of tree diebacks for Scots pine. This indicator rose from 8% in 2020 to 34% in 2021. This scoring will be continued in future years to monitor the health status of trees in the experiment.